《植物生理学报》 2012, 48(9): 874-880

青枯病病菌对茄子相关防卫信号基因表达及活性氧含量的影响

肖熙鸥*, 李冠男*, 曹必好**, 雷建军, 陈清华, 陈国菊

华南农业大学园艺学院, 广州510642

通信作者：曹必好；E-mail: caobh01@163.com；Tel: 020-85280228

摘　要：

本文以茄子抗青枯病自交系和感病自交系为试材, 在接种青枯病菌后, 对调控抗性反应的不同信号传导基因进行表 达特性分析。结果表明, 在9个基因中, EDS1、PAD4、NPR1、SGT1和WRYK70等5个基因的表达均随着接种诱导时间延长 而增加, 而且在抗病自交系‘E-31’中的表达量要高于感病自交系‘E-32’; 而JAR1、NDR1、EIN2和RAR1等基因的表达随着 接种诱导时间的增加, 表达水平变化不大。初步推断EDS1、PAD4、NPR1、SGT1和WRYK70可能与调节茄子抗青枯病抗 性反应有关。同时茄子在接种后活性氧的含量均增加, 但是感病植株的含量高于抗病植株。

关键词：茄子; 信号基因; 调控抗青枯病反应; 活性氧

收稿：2012-04-10   修定：2012-07-11

资助：国家自然科学基金(30972005)、广东省自然科学基金 (9151064201000063)和广东省自然科学基金研究团队项目 (S2011030001410)

Effect of Inoculation of Ralstonia solanacearum on Gene Expression in Defense Signaling Pathways and Accumulation of ROS in Eggplants

Xiao Xi-ou*, Li Guan-Nan*, Cao Bi-Hao**, LEi Jian-Jun, CHEN Qing-Hua, CHEN Guo-Ju

Horticulture College, South China Agriculture University, Guangzhou 510642, China

Corresponding author: Xiao Xi-ou; E-mail: caobh01@163.com; Tel: 020-85280228

Abstract:

The inbred lines ‘E-31’ (resistant to bacterial wilt ) and ‘E-32’ (susceptible to bacterial wilt) of eggplant (Solanaceae melongena L.) were induced after inoculation of Ralstonia solanacearum. The expressions of nine genes in signal pathway of disease resistance were identified in the R/S inbred lines of eggplant (Solanum melongena), such as EDS1, PAD4, NPR1, SGT1, WRYK70, JAR1, NDR1, EIN2, RAR1. The results showed that the expression levels of EDS1, PAD4, NPR1, SGT1, WRYK70 increased with the addition of induction time, and their levels of expression were higher in resistant inbred line ‘E-31’ than those in susceptible inbred line ‘E-32’, but the levels of expression of others four genes didn’t change in both resistance and susceptibility materials. The results deduced that five genes (EDS1, PAD4, NPR1, SGT1, WRYK70) might be related to regulation the resistance to bacterial wilt in eggplant. The contents of ROS were higher in ‘E-31’and ‘E-32’ after inoculation, and the content of ROS in susceptible plant was higher than that in resistant plant.

Key words: eggplant (Solanum melongena); signal gene; regulation resistance; ROS